Enzymatic Catalysis: Measurements and Mechanisms
Explore an introduction to the study of enzymatic catalysis, the process by which enzymes facilitate biochemical reactions in living organisms.
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Course Description
- Explore the fundamental role of enzymes in biochemical reactions and their industrial applications.
- Understand protein structure and the basics of enzymatic catalysis.
- Delve into unimolecular reactions, including deriving the Michaelis Menten equation and analyzing kinetic parameters.
- Analyze multi-reactant mechanisms and the engineering of enzymes, culminating in assessing enzyme performance in various applications.
What You Will Learn
By the end of this course, learners will be able to:
Comprehend the structure, function, and kinetics of enzymes, including both single and multi-reactant kinetics.
Understand the role of enzymes in biofuels, bioenergy, and the principles of enzyme inhibition and metabolism regulation.
Analyze enzyme specificity, including cofactor specificity, and the impact of mutations on substrate specificity.
Evaluate and compare engineered enzymes using innovative methods that factor in enzyme and reactant concentrations.
Course Outline
Module 1: Course introduction and overview
Module 2: Proteins and enzymes
Module 3: Enzymatic catalysis
Module 4: Single reactant kinetics
Module 5: Multi-reactant kinetics
Module 6: Enzyme example: alcohol dehydrogenase D
Module 7: How we compare engineered enzymes
Instructors
Scott Banta is the Chair and Professor of Chemical Engineering at Columbia University. His research focuses on applying protein engineering, metabolic engineering, and synthetic biology tools to solve various problems in bioengineering. Banta's work involves the modification of proteins and peptides to produce new molecules with novel functions, the characterization and improvement of enzyme networks to achieve new goals, and the design and creation of non-natural biological systems. His lab is currently working on engineering a unique beta-roll peptide sequence that can transition from an unfolded-state to a folded-state upon addition of calcium, developing new protein/protein and protein/nanomaterial interactions for catalytic processes, and creating biochemicals from CO2 using renewable electricity. Banta earned a BSE in chemical engineering from the University of Maryland Baltimore County in 1997, and a MS and PhD in chemical engineering from Rutgers University in 2000 and 2002, respectively. He began teaching at Columbia Engineering in 2004 and recently co-founded Ironic Chemicals LLC to create biofuels using copper mining waste.
Please note that there are no instructors or course assistants actively monitoring this course.